The antiserum was produced against synthesized peptide derived from human Caspase 9.
Immunogen Range
281-330
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
Caspase 9 (Cleaved-Asp330) Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp330.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CASP9
Database Links
Gene ID: 842 Swiss-Prot #: P55211
OMIM #: 602234 Unigene #: Hs.329502
Target Modification
Cleaved
Modification Sites
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp330) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2
Western blot analysis of lysates from HeLa cells, treated with Etoposide 25uM 60', using Caspase 9 (Cleaved-Asp330) Antibody. The lane on the right is blocked with the synthesized peptide.