Cy?3 Streptavidin is prepared by covalently labeling streptavidin with Cy?3 dye. Streptavidin has high binding affinity with biotin, so streptavidin conjugate is commonly used together with biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules. Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.
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