Description:
BacteriophageSP6RNAPolymeraseisaDNA-dependentRNApolymerasethatishighlyspecificfortheSP6phagepromoter.The98.5KDpolymerasecatalyzesinvitroRNAsynthesisfromaclonedDNAtemplateundertheSP6promoter.RNAsynthesizedusingtheSP6RNAPolymeraseissuitableformanyapplicationsinresearchandbiotechnology.
ReactionConditions:
1XRNAPolReactionBuffer,supplementedwith0.5mMeachATP,UTP,GTP,CTPandDNAtemplatecontainingtheSP6RNAPolymerasepromoter.Incubateat37°C.?
Highlights
- Isolatedfromarecombinantsource
- RNAprobepreparationforhybridization
- mRNAgenerationforinvitrotranslationsystems
- Suppliedwith10XReactionBuffer
ProductSource
An?E.coli?strainthatcarriestheclonedgeneforSP6RNAPolymerasefrom?Salmonellatyphimurium?LT2Z.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
RNAPolReactionBuffer | -20 | 10X |
Notes:
ForrADIolabeledhighspecificactivityRNAprobes,theconcentrationoftheradioactivenucleotideshouldbelimitedto6?μM.?ToprotectRNAagainstribonuclease,RNaseinhibitor(NEB#M0314or#M0307)shouldbeaddedtoafinalconcentrationof1U/μl.?SP6RNAPolymeraseisextremelysensitivetosaltinhibition.Theoverallsaltconcentrationshouldnotexceed50mM.?SP6RNAPolymeraseisslightlymoreactiveat40°Cthanat37°C.Incubationat40°CmaybeconsideredforRNAtranscriptcontainingstrongsecondarystructures.