DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3"→ 5" exonuclease activity, but has lost 5"→ 3" exonuclease activity (1). Klenow retains the polymerization fidelity of the holoenzyme without degrading 5" termini.
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An E. coli strain that contains the E. colipolA gene that has had its 5"→3" exonuclease domain removed.
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DNA Manipulation Products
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