Description:
ShortCut?RNaseIII,usedwithitsmanganese-containingreactionbuffer,convertslongdouble-strandedRNAintoaheterogeneousmixofshort(18–25bp)interferingRNAs(siRNA)suitableforRNAinterferenceinmammaliancells(1–3).1.5units(1?μl)ofShortCutRNaseIIIissufficienttoconvert1μgofdsRNAintosiRNAsuitableforRNAinterferenceinmammaliancells.
Highlights
- Isolatedfromarecombinantsource
- Genesilencing
- Targetvalidation
- Suppliedwith10XReactionBuffer
ProductSource
AnE.colistraincontainingageneticfusionoftheE.coliRNaseIIIgene(rnc)andthegenecodingformaltosebindingprotein(MBP).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
10XEDTA | | 500mM |
MnCl2 | | 10X |
GlycogenRNase-Free | | 10mg/ml |
ShortcutReactionBuffer | | 10X |