Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´ → 3´ polymerase activity, but lacks 5´ →3´ exonuclease activity.
Highlights
Isolated from a recombinant source
Sequencing through problematic secondarystructures
Supplied with 10X Reaction Buffer
Product Source
BstPolymerase, Large Fragment is prepared from anE. colistrain containing a genetic fusion of theBacillus stearothermophilusDNA Polymerase gene, lacking the 5´ → 3´ exonuclease domain, and the gene coding forE. colimaltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion of the fusion is cleaved offin vitro. The remaining polymerase is purified free of MBP (1).
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