Description:
AntarcticPhosphatase(AnP)isaheatlABIlealkalinephosphatasepurifiedfromarecombinantsource.AnPnonspecificallycatalyzesthedephosphorylationof5′and3′endsofDNAandRNAphosphomonoesters.Also,AnPHydrolysesribo-,aswellasdeoxyribonucleosidetriphosphates(NTPsanddNTPs).AnPisusefulinmanymolecularBIOLOGyapplicationssuchastheremovalofphosphorylatedendsofDNAandRNAforsubsequentuseincloningorend-labelingofprobes.Incloning,dephosphorylationpreventsreligationoflinearizedplasmidDNA.Theenzymeactson5′protruding,5′recessed,andbluntends.AnPmayalsobeusedtodegradeunincorporateddNTPsinPCRreactionstopreparetemplatesforDNAsequencingorSNPanalysis.AnPiscompletelyandirreversIBLyinactivatedbyheatingat80°Cfor2minutes,therebymakingremovalofAnPpriortoligationorend-labelingunnecessary.?
ProductSource
An?E.coli?strainthatcarriestheTAB5APgene,originallyclonedinplasmidpNI(2),reclonedinplasmidpEGTAB7-4.1(3).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
AntarcticPhosphataseReactionBuffer | -20 | 10X |
Notes:
Adding1/10volumeof10XAntarcticPhosphataseReactionBufferwillprovidetheamountofZn2+?thattheenzymerequiresforactivity.