Description:
TmaEndonucleaseIIIisaThermostablehomologofE.coliEndonucleaseIII,theproductoftheE.colinthgene(1).ItpossessesbothN-glycosylaseandAP-lyaseactivities.TheN-glycosylaseactivityreleasesdamagedpyrimidinesfromdouble-strandedDNA,generatinganabasicsite.TheAP-lysaseactivitycleavesthephosphodiesterbond3′totheabasicsiteresultingina3′-α,β-unsaturatedaldehydeanda5′-phosphate.
Damagedbases?recognized?andremovedby?Tma?EndonucleaseIIIinclude?5,6?dihydroxythymine,thymineglycoland?abasicsites?(2).
ProductSource
ClonedfromThermotogamaritimaandoverexpressedinE.coli.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
ThermoPol® ReactionBufferPack | -20 | 10X |
Notes:
DiluentCompatibility:?DiluentD100mMKCl,10mMTris-HCl(pH7.4@25°C),0.1mMEDTA,1mMDTT,0.1%TritonX-100and50%glycerol.