Description:
EndonucleaseVisarepairenzymefoundinE.colithatrecognizesdeoxyinosine,adeaminationproductofdeoxyadenosineinDNA.EndonucleaseV,oftencalleddeoxyinosine3′?endonuclease,recognizesDNAcontainingdeoxyinosines(pairedornot)ondouble-strandedDNA,single-strandedDNAwithdeoxyinosinesandtoalesserdegree,DNAcontainingabasicsites(ap)orurea,basemismatches,insertion/deletionmismatches,hairpinorunpairedloops,flapsandpseudo-Ystructures.ItisbelievedthatEndonucleaseVneedsanotherproteintorepairtheDNA,asitdoesnotremovethedeoxyinsoineorthedamagedbases(1,2,3).
EndonucleaseVcleavesthesecondphosphodiesterbonds3′tothemismatchofdeoxyinosine(2),leavinganickwith3′-hydroxyland5′-phosphate(4).
Highlights
- Isolatedfromarecombinantsource
- Suppliedwith10XReactionBuffer
ProductSource
AnE.colistraincontainingagenefusionoftheEndoVgeneandthegenecodingforthemaltosebindingprotein(MBP).Thefusionproteinispurifiedtonearhomogeneityandisactiveasafusion.Theproteincontains223aminoacidsandhasamolecularweightof24.9kDa(5).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBuffer™4 | -20 | 10X |