Bsu DNA Polymerase I, Large Fragment retains the 5´→ 3´ polymerase activity of the Bacillussubtilis DNA polymerase I (1), but lacks the 5´→ 3´ exonuclease domain. This large fragment naturally lacks 3´→ 5´ exonuclease activity.
Product Source
AnE. colistrain that contains a genetic fusion of theBacillus subtilisDNA polymerase I gene (starting from codon 297 thus lacking the 5´→ 3´ exonuclease domain), and the gene coding for maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion is cleaved offin vitro. The remaining DNA polymerase is purified free of MBP.
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DNA Manipulation Products
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