Description:
OneTaq?2XMasterMixwithStandardBufferisanoptimizedblendofTaqandDeepVentR?DNAPolymerasesideallysuitedtoroutinePCRapplicationsfromavarietyoftemplatesincludingpureDNAsolutions,bacterialcolonies,andCDNAproducts.The3′→5′exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).TheconvenientmastermixformulationcontainsdNTPs,MgCl2,buffercomponentsandstABIlizers,requiringonlytheadditionofprimersandDNAtemplateforrobustamplification.
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AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.Notes:
OneTaq2XMasterMixwithStandardBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaq2XMasterMixwithStandardBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.ProductspecificationsforindividualcomponentsintheOneTaq2XMasterMixwithStandardBufferareavailableseparately.