Description:
OneTaq?HotStart2XMasterMixwithGCBufferisanoptimizedblendofTaqandDeepVent?DNAPolymerasescombinedwithanaptamer-basedinhibitor.ThisenzymeblendisideallysuitedtoPCRapplicationsfromGC-richtemplates,includingpureDNAsolutions,bacterialcoloniesandCDNAproducts.The3′→5′exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Thehotstartnatureoftheenzymeoffersconveniencewithdecreasedinterferencefromprimerdimersandsecondaryproducts.TheconvenientmastermixformulationcontainsdNTPs,MgSO4,?buffercomponentsandstABIlizers,requiringonlytheadditionofprimersandDNAtemplateforrobustamplification.
??ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andan?E.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
OneTaq?HighGCEnhancer | | 50% |
Notes:
OneTaqHotStart2XMasterMixwithGCBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaqHotStart2XMasterMixwithGCBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.ProductspecificationsforindividualcomponentsintheOneTaqHotStart2XMasterMixwithGCBufferareavailableseparately.