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NEB/OneTaq? Quick-Load? 2X Master Mix with Standard Buffer/500 reactions (50 μl)/M0486L

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貨號(hào): M0486L

品牌: NEB

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- OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep Vent^®DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3′→5′ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient quick-load master mix formulation contains dNTPs, MgCl₂, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.
  Comparison of OneTaq Products to Other Commercially Available Polymerases
  Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip^®. A large, dark green dot represents the highest yield and purity.
  Product Source
  An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent^® DNA Polymerase gene from Pyrococcus species GB-D.
  This product is related to the following categories:
  OneTaq® DNA Polymerases Products,
  Taq DNA Polymerase Products,
  Master Mixes Products
  This product can be used in the following applications:
  Multiplex PCR,
  Specialty PCR,
  Routine PCR,
  PCR
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