Description:
OneTaq?HotStartQuick-Load?2XMasterMixwithStandardBufferisanoptimized,ready-to-useblendofTaqandDeepVentR?DNAPolymerasescombinedwithanaptamer-basedinhibitor.ThisenzymeblendisideallysuitedtoroutinePCRapplicationsfromavarietyoftemplates,includingpureDNAsolutions,bacterialcoloniesandCDNAproducts.The3′→5′exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Thehotstartnatureoftheenzymeoffersconveniencewithdecreasedinterferencefromprimerdimersandsecondaryproducts.TheconvenientQuick-LoadmastermixformulationcontainsdNTPs,MgCl2,buffercomponentsandstABIlizersaswellastwocommonlyusedtrackingdyesforDNAgels.Ona1%agarosegelin1XTBE,XyleneCyanolFFmigratesat~4kbandTartrazinemigratesat~10bp.Bothdyesarepresentinconcentrationsthatdonotmaskco-migratingDNAbands.
??ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.Notes:
ProductspecificationsforindividualcomponentsintheOneTaqHotStartQuick-Load2XMasterMixwithStandardBufferareavailableseparately.OneTaqHotStartQuick-Load2XMasterMixwithStandardBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°COneTaqHotStartQuick-Load2XMasterMixwithStandardBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.