The Q5 High-Fidelity 2X MasterMix offers robust, high-fidelity performance in aconvenient master mix format. The Q5 High-Fidelity 2X Master Mix features a high-fidelity,thermostable DNA polymerase with 3´→ 5´exonuclease activity, fused to a processivity-enhancingSso7d domain to support robust DNAamplification. With an error rate ~280-fold lowerthan that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymeraseis ideal for cloning and can be used for long ordifficult amplicons. The convenient master mixformulation is supplied at a 2X concentration.The mix contains dNTPs, Mg++ and a proprietarybroad-use buffer requiring only the addition ofprimers and DNA template for robust amplificationregardless of GC content. When used at therecommended 1X final concentration, the Q5High-Fidelity Master Mix contains 2 mM Mg++.Q5 High-Fidelity DNA Polymerase is unlike typical,lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Product Source
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
This product is related to the following categories:
Q5® High-Fidelity DNA Polymerases Products,
Master Mixes Products
This product can be used in the following applications:
Long Range PCR,
Fast PCR,
High-Fidelity PCR,
Multiplex PCR,
Specialty PCR,
Routine PCR,
DNA Amplification, PCR & qPCR,
PCR,
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