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NEB/Q5? Hot Start High-Fidelity 2X Master Mix/500 reactions/M0494X

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貨號(hào): M0494X

品牌: NEB

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- The Q5^® Hot Start High-Fidelity 2XMaster Mix features a high-fidelity, thermostable,hot start DNA polymerase with 3´→ 5´ exonucleaseactivity, fused to a processivity-enhancing Sso7ddomain to support robust DNA amplification. Theaddition of an aptamer-based inhibitor allowsroom temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity2X Master Mix is ideal for cloning and can be usedfor long or difficult amplicons. The convenientmaster mix formulation is supplied at a 2Xconcentration. The mix contains dNTPs, Mg⁺⁺ anda proprietary broad-use buffer requiring only theaddition of primers and DNA template for robustamplification regardless of GC content. When usedat the recommended 1X final concentration, theQ5 Hot Start High-Fidelity Master Mix contains2 mM Mg⁺⁺. Q5 Hot Start High-Fidelity 2X MasterMix is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
  Q5-High Fidelity 2X Master Mix formats allow robust amplification of a broad range of targets with a single formulation.
  Amplification of a variety of human genomic amplicons from low to high GC content using Q5 Hot Start High-Fidelity 2X Master Mix. All reactions were set up at room temperature, conducted using 30 cycles of amplification and visualized by microfluidic LabChip^® analysis.
  Product Source
  An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
  This product is related to the following categories:
  Q5® High-Fidelity DNA Polymerases Products,
  Master Mixes Products
  This product can be used in the following applications:
  Site Directed Mutagenesis,
  Site Directed Mutagenesis,
  High-throughput cloning and automation solutions,
  Hot Start PCR,
  Long Range PCR,
  Fast PCR,
  High-Fidelity PCR,
  Multiplex PCR,
  Specialty PCR,
  Routine PCR,
  DNA Amplification, PCR & qPCR,
  PCR,
  Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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