Hot Start Taq DNA Polymeraseis a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity1,2,3 and a 5´ flap endonuclease activity4,5.It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1. - This product is related to the following categories:
- Taq DNA Polymerase Products
- This product can be used in the following applications:
- Colony PCR,
- Multiplex PCR,
- Specialty PCR,
- Routine PCR,
PCR