Hot Start Taq 2X Master Mix is anoptimized ready-to-use solution containing HotStart Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is ideallysuited to routine PCR applications from templatesincluding pure DNA solutions, bacterial colonies,and cDNA products. It is recommended foramplification up to 5 kb.Hot Start Taq DNA Polymerase is a mixture of TaqDNA Polymerase and an aptamer-based inhibitor.The inhibitor binds reversibly to the enzyme,inhibiting polymerase activity at temperaturesbelow 45°C, but releases the enzyme duringnormal cycling conditions allowing reactions tobe set up at room temperature. The aptamer-basedhot start does not require a separate hightemperature incubation step to activate the enzyme.Hot Start Taq DNA Polymerase possesses a5´→ 3´ polymerase activity1,2,3 and a 5´ flapendonuclease activity.4,5
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
This product is related to the following categories:
Taq DNA Polymerase Products,
Master Mixes Products
This product can be used in the following applications: