Description:
LongAmpHotStartTaq2XMasterMixcontainsauniqueblendofaptamer-basedHotStartTaqandDeepVentR?DNAPolymerases.Theaptamer-basedinhibitorbindsreversIBLytotheenzyme,inhibitingpolymeraseactivityattemperaturesbelow45°C,butreleasestheenzymeduringnormalPCRcyclingconditions.ThispermitsassemblyofPCRreactionsatroomtemperature.TheLongAmp?HotStartTaq2XMasterMixdoesnotrequireaseparatehightemperatureincubationsteptoactivatetheenzyme.The3′→5′exonucleaseactivityofDeepVentRDNAPolymeraseincreasesthefidelityandrobustamplificationofHotStartTaqDNAPolymerase(1).LongAmp?HotStartTaqDNAPolymeraseofferstwo-foldhigherfidelitythanHotStartTaqDNAPolymerasealone.Theconvenientmastermixformulationissuppliedata2XconcentrationandcontainsdNTPsandMg++,requiringonlytheadditionofprimersandDNAtemplateforrobustamplification.AwiderangeofPCRproductscanbegenerated;upto30kbfromlamBDaorhumangenomicDNA.
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacidinsolublematerialin30minutesat75°C.
UnitAssayConditions:
1XThermoPol?ReactionBuffer,200μMdNTPsincluding[3H]-dTTPand200?μg/mlactivatedCalfThymusDNA.
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
LongAmp??TaqReactionBufferPack | -20 | 5X |
Notes:
ProductspecificationsforindividualcomponentsintheLongAmpHotStartTaq?2XMasterMixareavailableseparately.