當(dāng)前位置 ： NEB >>> NEB/Faustovirus Capping Enzyme/2,500 units/M2081L
NEB/Faustovirus Capping Enzyme/2,500 units/M2081L
  • NEB/Faustovirus Capping Enzyme/2,500 units/M2081L

NEB/Faustovirus Capping Enzyme/2,500 units/M2081L

價(jià)格: 試用 市場(chǎng)價(jià): 0.00
貨號(hào): M2081L
品牌: NEB
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產(chǎn)品：無質(zhì)量問題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問
咨詢顧問

全國免費(fèi)服務(wù)熱線

4000-520-616

  • 自營商城 一站式服務(wù)
  • 廠家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Faustovirus Capping Enzyme (FCE) catalyzes the addition of N7-methylguanosine cap (m7G) to the 5′ end of triphosphorylated and diphosphorylated transcripts, producing Cap-0 RNA(1). FCE is a single-subunit enzyme that combines the three activities necessary to produce the Cap-0 structure- triphosphatase, guanylyltransferase, and (guanine-N7)-methyltransferase. Installation of Cap-0 is a key step in eukaryotic mRNA maturation along with 2′-O-methylation at position 1 (Cap-1) and polyadenosine (poly(A)) tailing. The Cap-0 structure also promotes RNA stability(2) and prevents inadvertent activation of innate immune responses triggered by triphosphorylated RNA(3).FCE retains significant capping activity at low temperatures and tolerates reaction temperatures up to 55°C. In many cases, 1 µl of FCE (25 units) can cap over 100 µg of RNA in 1 hour at 37°C. GTP and S-adenosylmethionine (SAM) are required for capping activity and are included with the enzyme.Source: An E. coli strain that carries a plasmid encoding the Faustovirus Capping Enzyme with a C-terminal His-tag.
      Figure 1: FCE offers increased capping efficiency and workflow optimizationFigure 1
      A. mRNA capping by FCE and Vaccinia Capping Enzyme (VCE) at 37°C. 200 μg (~350 picomoles, 7 μM) of a 1.77 kb FLuc transcript having 5´-UTR sequences as indicated were treated with a limiting amount of FCE (25 units, 1 picomole, 20 nM in 50 μl) or VCE (10 units, 1 picomole, 20 nM in 50 μl) for 1 hour at 37°C. Note that this is less than our recommended amount of enzyme highlighting the increased capping efficiency of FCE vs VCE and the potential benefits of workflow optimization.B. mRNA capping by FCE at 37°C and 42°C. 200 μg (~350 picomoles, 7 μM) of a 1.77 kb FLuc transcript having 5′-UTR sequences as indicated were treated with a limiting amount (25 units, 1 picomole or 20 nM) of FCE for 1 hour at 37°C or 42°C. Note that this is less than our recommended amount of enzyme highlighting the potential benefits of workflow optimization. All capping reactions were performed in 50 μl reactions containing 0.1 mM SAM, and 0.5 mM GTP, 1X FCE Capping Buffer for FCE reactions or 1X Capping Buffer for VCE reactions. Following capping reactions, mRNA capping was measured using targeted RNase H cleavage and LC-MS.
      Figure 2: FCE expands temperature range for RNA capping reactionsFigure 2
      RNA capping by FCE and VCE from 15ºC to 60ºC. Capping reactions (20 μl) contained 2.5 μM RNA substrate (5´-triphosphate 20mer 3´-FAM), a limiting concentration (8 nM) of capping enzyme, 0.1 mM SAM, and 0.5 mM GTP. FCE capping reactions were performed in 1X FCE Capping Buffer, and VCE capping reactions were performed in 1X Capping Buffer. All reactions were incubated for 30 minutes at the indicated reaction temperature. Reaction products were measured by capillary electrophoresis.
      Figure 3. FCE offers robust capping of a variety of RNA 5´ ends (at least 100 μg yield per 50 units)Figure 3
      Extent of mRNA capping by FCE. 200 μg (~350 picomoles, 3.5 uM) of a 1.77 kb FLuc transcript having 5´-UTR sequences as indicated were treated with 2 μl (50 units, 2 picomoles, 20 nM) of FCE in 100 μl reactions containing 1X FCE buffer for 1 hour at 37°C. Following capping reactions, mRNA capping was measured using targeted RNase H cleavage and LC-MS.
      This product is related to the following categories:
      RNA Modification Products,
      RNA Reagents Products,
      RNA Synthesis Products
      This product can be used in the following applications:
      RNA Modification,
      In vitro Transcription for RNA Synthesis,
      RNA Analysis
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專業(yè)人員負(fù)責(zé)品控、采購、物流、銷售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠家，從全國各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營商城，直接從廠家采購， 自己的團(tuán)隊(duì)負(fù)責(zé)國際物流和清關(guān)，中間沒有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話：4000-520-616

    Q：可以開發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問題夠可以電話咨詢我們，全國24小時(shí)免費(fèi)服務(wù)熱線：4000-520-616 或聯(lián)系我們的在線客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購買多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線客服或者致電4000-520-616。

    Q：退換貨/維修需要多長時(shí)間？
    A：一般情況下，退貨處理周期為客戶收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡而嚴(yán)謹(jǐn)，我們僅銷售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢梗劢拐邕x每一款產(chǎn)品，聚焦服務(wù)每一位客戶!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知