Mouse monoclonal antibody raised against partial recombinant NGFR.
Immunogen:
Recombinant protein corresponding to human NGFR.
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (1:10000)Western Blot (1:500-1:2000)Immunofluorescence (1:200-1:1000)Flow cytometry (1:200-1:400)The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Applications
Western Blot (Transfected lysate)
Western blot analysis using NGFR monoclonal antibody, clone 2F1C2 (Cat # MAB10831) against NGFR-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence
Immunofluorescence analysis of EC cells (rat aortic endothelial cells) using NGFR monoclonal antibody, clone 2F1C2 (Cat # MAB10831) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of EC cells (rat aortic endothelial cells) using NGFR monoclonal antibody, clone 2F1C2 (Cat # MAB10831) (green) and negative control (purple).
Application Image
Western Blot (Transfected lysate)
enlarge
Immunofluorescence
enlarge
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
enlarge
Gene Information
Entrez GeneID:
4804
Gene Name:
NGFR
Gene Alias:
CD271,Gp80-LNGFR,TNFRSF16,p75(NTR),p75NTR
Gene Description:
nerve growth factor receptor (TNFR superfamily, member 16)
Omim ID:
162010
Gene Ontology:
Hyperlink
Gene Summary:
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq