ApplicationNotes | Indirectimmunofluorescence(1:100-1:500)inthedetectionofneuronsintissueculture.
UsingdoubleimmunofluorescencetheGQgangliosidehasasimilardistributiononneuronstotheD2protein,tothetetanustoxinreceptorandtoneurofilaments(Walsh,1980).
Mayalsobeusedinthedepletionofneuronsforamixedpopulationortheirpurificationbyaffinitychromatography.
Mayalsobeusefulfordetectingthemetastaticspreadofneuroblastomacellsintobonemarrow.
Flowcytometry:livecells{Maric,D.etal.(2000)CerebralCortex10:729-747}.
Immunohistochemistry:Frozen,fixedtissues(Levison&McCarthy,1989)
Complement-mediatedcytotoxicity(Eisenbarthetal.,1979)
Optimalworkingdilutionsmustbedeterminedbyenduser. |