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Millipore/MAB3199 | Anti-E-Cadherin Antibody, clone 67A4/MAB3199/100 µg
  • Millipore/MAB3199 | Anti-E-Cadherin Antibody, clone 67A4/MAB3199/100 µg

Millipore/MAB3199 | Anti-E-Cadherin Antibody, clone 67A4/MAB3199/100 µg

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貨號: MAB3199
品牌: Millipore
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    • Description
      CatalogueNumberMAB3199
      Replaces04-1103
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-E-CadherinAntibody,clone67A4
      AlternateNames
      • CD324
      ProductInformation
      FormatPurified
      PresentationProteinApurifiedimmunoglobulin.Liquidin0.02MPBSwithNaCl,pH=7.6,0.1%Sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintain2-8°Cinundilutedaliquotsforupto12monthsafterdateofreceipt.
      Applications
      ApplicationAnti-E-CadherinAntibody,clone67A4detectslevelofE-Cadherin&hasbeenpublished&validatedforuseinFC&WB.
      KeyApplications
      • FlowCytometry
      • WesternBlotting
      • AffectsFunction
      ApplicationNotesImmunoblotting:123kDinmembranefractions;MCF-7membranespositivecontrol.Confluentcellswerewashedthreetimeswithice-coldPBSandlysedin50mMHEPESpH7.5,150mMNaCl,1mMEDTA,10%glycerine,1%tritonX-100,20mMNa(4)P(2)O(7),2mMNa-vanadate,100mMNaF,2mMPMSF,20μg/mLleupeptin,1mMpNPP(para-nitrophenylphosphate),20μg/mLaprotinin,and200μMNH(4)-molyBDate.Crudeextractswereclarifiedbycentrifugation(10,000xg10min),andsupernatantsweretakenforimmunoprecipitation.ProteinconcentrationofclearedlysatesweredeterminedbyBCAproteinassay.{Buhring,etalLuekemia10:106-116,1996}.



      FACSAnalysis

      FunctionalblockingofE-cadherin

      Doesnotworkwellforimmunoprecipitation

      Originalpaper,Buhring,HetalLeukemia10:106-116,1996.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenT-47Dbreastcarcinomaline
      Clone67A4
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityAntibodiesagainstE-cadherinaresuitabletocharacterizeepithelialcells.E-Cadherinisinvolvedinadhesionofepithelialcells(mostlyhomotypicandhomophilic).E-cadherinisalsoexpressedonimmatureerythroidcells.Theantibody67A4issuitabletostudyE-cadherinexpressionbyflowcytometry.Inaddition,itblockstheinteractionofE-cadherinmoleculesandthereforeissuitabletostudyE-cadherin-mediatedsignaltransduction.

      Antigenisexpressedinnon-neuralepithelialtissues.
      IsotypeIgG1
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisaclassicalcadherinfromthecadherinsuperfamily.Theencodedproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Mutationsinthisgenearecorrelatedwithgastric,breast,colorectal,thyroidandovariancancer.Lossoffunctionisthoughttocontributetoprogressionincancerbyincreasingproliferation,invasion,and/ormetastasis.Theectodomainofthisproteinmediatesbacterialadhesiontomammaliancellsandthecytoplasmicdomainisrequiredforinternalization.Identifiedtranscriptvariantsarisefrommutationatconsensussplicesites.
      GeneSymbol
      • CDH1
      • CDHE
      • E-cadherin
      • Arc-1
      • Uvomorulin
      • UVO
      • Cadherin-1
      • CD324
      • uvomorulin
      • ECAD
      • LCAM
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P12830#E-Cad/CTF2promotesnon-amyloidogenicdegradationofAbetaprecursors.HasastronginhibitoryeffectonAPPC99andC83production.
      SIZE:882aminoacids;97456Da
      SUBUNIT:Homodimer;disulfide-linked.Interactsdirectly,viathecytoplasmicdomain,withCTNNB1orJUPtoformthePSEN1/cadherin/cateninadhesioncomplexwhichconnectstotheactinskeletonthroughtheactinbindingofalpha-catenin.InteractionwithPSEN1,cleavesCDH1resultinginthedisassociationofcadherin-basedadherensjunctions(CAJs).InteractswithAJAP1,CTNND1andDLG7.
      SUBCELLULARLOCATION:Celljunction.Cellmembrane;Single-passtypeImembraneprotein.Note=ColocalizeswithDLG7atsitesofcell-cellcontactinintestinalepithelialcells.AnchoredtoactinMICROFILamentsthroughassociationwithalpha-,beta-andgamma-catenin.Sequentialproteolysisinducedbyapoptosisorcalciuminflux,resultsintranslocationfromsitesofcell-cellcontacttothecytoplasm.
      TISSUESPECIFICITY:Non-neuralepithelialtissues.
      PTM:Duringapoptosisorwithcalciuminflux,cleavedbyamembrane-boundmetalloproteinase(ADAM10),PS1/gamma-secretaseandcaspase-3toproducefragmentsofabout38kDa(E-CAD/CTF1),33kDa(E-CAD/CTF2)and29kDa(E-CAD/CTF3),respectively.Processingbythemetalloproteinase,inducedbycalciuminflux,causesdisruptionofcell-celladhesionandthesubsequentreleaseofbeta-cateninintothecytoplasm.Theresidualmembrane-tetheredcleavageproductisrapidlydegradedviaanintracellularproteolyticpathway.Cleavagebycaspase-3releasesthecytoplasmictailresultingindisintegrationoftheactinmicrofilamentsystem.Thegamma-secretase-mediatedcleavagepromotesdisaaaemblyofadherensjunctions.
      DISEASE:"SwissProt:P12830#DefectsinCDH1areinvolvedindysfunctionofthecell-celladhesionsystem,triggeringcancerinvasion(gastric,breast,ovary,endometriumandthyroid)andmetastasis.&DefectsinCDH1areacauseofhereditarydiffusegastriccancer(HDGC)[MIM:137215].&DefectsinCDH1areacauseofsusceptibilitytoendometrialcancer[MIM:608089]."
      SIMILARITY:SwissProt:P12830##Contains5cadherindomains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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