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Millipore/MAB345 | Anti-O4 Antibody, clone 81/MAB345/50 µg
  • Millipore/MAB345 | Anti-O4 Antibody, clone 81/MAB345/50 µg

Millipore/MAB345 | Anti-O4 Antibody, clone 81/MAB345/50 µg

價(jià)格: ￥4440.00 市場(chǎng)價(jià): 7400.00
貨號(hào): MAB345
品牌: Millipore
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    • Description
      CatalogueNumberMAB345
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-O4Antibody,clone81
      AlternateNames
      • Sulfatide
      BackgroundInformationOligodendrocytesandastrocytesarederivedfromcommonprecursorcells,glioblasts(Sommer,1981).O-Antigensaresulfatides,whichfunctionasdifferentiationMarkersonthesurfaceofoligodendrocytesofthecentralnervoussystem.O4isformedpostnatally(Schachner,1981)andisamarkerforcellbodiesandprocessesofoligodendrocytestypesIandII(hairyeyeballtype).Duringoligodendrocytedifferentiation,O4occursinpro-oligodendrocytes,butnotinO-2A-Progenitorcells.O4occursfromday3onwardsincellculturesofembryonicmousebrain.Anti-O4canbeusedinmyelination,demyelinationandremyelinationstudiesandinregenerationexperiments.
      ProductInformation
      FormatPurified
      Control
      • Ratcorticalstemcellsorday3cellculturesofbrainsfrommouseembryos
      PresentationPurifiedimmunoglobulinin0.05MPotassiumphosphatebuffer,pH8.0with0.3MNaCland0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat2–8°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationAnti-O4Antibody,clone81isanantibodyagainstO4foruseinIC,IHwithmorethan50productcitations.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      ApplicationsNotRecommended
      • Immunoprecipitation
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:10-20μg/mLonunfixed,shockfrozentissue.

      Immunocytochemistry:10-20μg/mLoncellsfixedwith4%paraformaldehyde.

      Note:O4isasulfatide,whichcanbedissolvedoutofthemembranebyorganicsolvents;acetoneandmethanolshouldnotbeusedforfixation.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      Immunohistochemistryprotocol

      1.Preparesectionsfromunfixed,shockfrozentissue.Thesectionsshouldbe4-5μmthick.Placethesectionsonmicroscopeslides.

      2.Washtheslidethreetimesfor5min.eachinPBSatroomtemperature.

      3.Blockthenon-specificbindingsitesbyincubatingthesectionsinahumidchamberwith5%FCSatroomtemperaturefor30minutes.

      4.Washtheslidesasdescribedinstep2.

      5.CoverthesectionswithasufficientamountofMAB345(10-20μg/mLinPBS)andincubateinahumidchamberat37°Cforonehour.

      6.WashtheslidesbrieflythreetimeswithPBS.Carefullydryaroundtheareatobestained.

      7.Coverthesectionswithasufficientamountofanti-mouseIgM-fluorescein*solutionandincubateinahumidchamberat37°Cforonehour.

      8.Washtheslidesasdescribedinstep6.

      9.Coverthesectionswithasuitableembeddingmedium,coverwithacoverslip,andexaminebyfluorescencemicroscopy.



      *HRPorABCcanalsobeused.



      Optimalresultscanbeobtainedbytitratingtheprimaryandsecondaryantibodies



      Immunocytochemistry



      1.Fixthepreparationswith4%paraformaldehyde(inPBS)atroomtemperaturefor10minutes.O4isasulfatidewhichcanbedissolvedoutofthemembranebyorganicsolvents;acetoneandmethanolshouldnotbeusedforfixation.

      2.Washtheslidethreetimesfor5min.eachinPBSatroomtemperature.

      3.Blockthenon-specificbindingsitesbyincubatingthesectionsinahumanchamberwith5%FCSatroomtemperaturefor30minutes.

      4.Washtheslidesasdescribedinstep2.

      5.CoverthesectionswithasufficientamountofMAB345(10-20μg/mLinPBS)andincubateinahumidchamberat37°Cforonehour.

      6.WashtheslidesbrieflythreetimeswithPBS.Carefullydryaroundtheareatobestained.

      7.Coverthesectionswithasufficientamountofanti-mouseIgM-fluoresceinsolutionandincubateinahumidchamberat37°Cforonehour.

      8.Washtheslidesasdescribedinstep6.

      9.Coverthesectionswithasuitableembeddingmedium,coverwithacoverslip,andexaminebyfluorescencemicroscopy.



      Note:Donotallowthepreparationstodryoutduringstaining.
      BIOLOGicalInformation
      ImmunogenHomogenateofwhitematterofcorpuscallosumfrombovinebrain.
      Clone81(alsoreferredtointheliteratureasmABO4)
      ConcentrationVaries,seelotspecificCoA
      HostMouse
      SpecificityRecognizesOligodendrocytemarkerO4.Alsoreactswithcertaingalactolipidsinsperm(seeAdditionalInformationlibraryforlist).
      IsotypeIgM
      SpeciesReactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      AntibodyTypeMonoclonalAntibody
      PurificationMethodAmmoniumSulfatePrecipitation
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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