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Millipore/MAB5254 | Anti-Neurofilament 160 kDa Antibody, clone NN18/MAB5254/40 µg
  • Millipore/MAB5254 | Anti-Neurofilament 160 kDa Antibody, clone NN18/MAB5254/40 µg

Millipore/MAB5254 | Anti-Neurofilament 160 kDa Antibody, clone NN18/MAB5254/40 µg

價格: ¥3816.00 市場價: 6360.00

貨號: MAB5254
品牌: Millipore
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    • Description
      CatalogueNumberMAB5254
      ReplacesCBL214
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Neurofilament160kDaAntibody,cloneNN18
      BackgroundInformationNeurofilamentsareatypeofintermediatefilamentthatserveasmajorelementsoftheCytoskeletonsupportingtheaxoncytoplasm.Theyarethemostabundantfibrillarcomponentsoftheaxon,beingonaverage3-10timesmorefrequentthanaxonalmicrotubules.Neurofilaments(10nmindia.)arebuiltfromthreeintertwinedprotofibrilswhicharethemselvescomposedoftwotetramericprotofilamentcomplexsofmonomericproteins.Theneurofilamenttripletproteins(68/70,160,and200kDa)occurinboththecentralandperipheralnervoussystemandareusuallyneuronspecific.The68/70kDaNF-Lproteincanself-assembleintoafilamentousstructure,howeverthe160kDaNF-Mand200kDaNF-Hproteinsrequirethepresenceofthe68/70kDaNF-Lproteintoco-assemble.Neuromas,ganglioneuromas,gangliogliomas,ganglioneuroblastomasandneuroblastomasstainpositivelyforneurofilaments.Althoughtypicallyrestrictedtoneurons,neurofilamentshavebeendetectedinparagangliomasandadrenalandextra-adrenalpheochromocytomas.Carcinoids,neuroendocrinecarcinomasoftheskin,andoatcellcarcinomasofthelungalsoexpressneurofilaments.FormoreneurofilamentinformationseeNervousSystemCellTypeSpecificMarkerchartonlineundertheCHEMICONTechnicalSupportsection.
      ProductInformation
      FormatPurified
      Control
      • Brain
      • Culturedneurons
      PresentationPurifiedmousemonoclonalIgG1inbuffercontaining0.02MPhosphatebuffer,0.25MNaClwith0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor6monthsat2-8oCinundilutedaliquotsfromdateofreceipt.
      Donotfreeze.
      Applications
      ApplicationAnti-Neurofilament160kDaAntibody,cloneNN18isanantibodyagainstNeurofilament160kDaforuseinIH,IH(P)&WB.
      KeyApplications
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:
      5-10μg/mLonfrozenorparaffinembeddedtissue.Seeprotocolsfordetails.

      WesternBlot:
      1:1000.7.5%-10%SDS-PAGE

      Optimalworkingdilutionsandprotocolsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenPurifiedneurofilamentpolypeptides.
      CloneNN18
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityTheantibodyreactswithneurofilament160kD(Debus,E.,1982;Shaw,G.,1984).
      IsotypeIgG1
      SpeciesReactivity
      • Chicken
      • GuineaPig
      • Mouse
      • Pig
      • Rat
      • Human
      SpeciesReactivityNoteExpectedtoreactwithhumanbasedonsequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • NEFM
      • NF-M
      • NEF3
      • NFM
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P07197#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber.
      SIZE:916aminoacids;102448Da
      PTM:ThereareanumberofrepeatsofthetripeptideK-S-P,NFMisphosphorylatedonanumberoftheserinesinthismotif.ItisthoughtthatphosphorylationofNFMresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.&Phosphorylationseemstoplayamajorroleinthefunctioningofthelargerneurofilamentpolypeptides(NF-MandNF-H),thelevelsofphosphorylationbeingaltereddevelopmentallyandcoincidentwithachangeintheneurofilamentfunction.
      SIMILARITY:SwissProt:P07197##Belongstotheintermediatefilamentfamily.
      MolecularWeight160kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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