Specificity | Acetylcholine.Byimmunohistochemistryonratbasalforbrainandspinalchordsections,theantibodyspecificallystainsnervecellbodiesandfibres(Chagnaud,1989).
Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds:
CompoundCross-reactivity
Choline-GA-BSA1
Choline-AA-BSA1/536
Choline-SA-BSA1/2,500
Farnesol-BSA1/>50,000
Retinoicacid-BSA1/>50,000
Myristicacid-BSA1/>50,000
Acetylcholine1/>50,000
Choline1/>50,000
Abbreviations:
(GA)Glutaricanhydride
(AA)ADIpicacid
(SA)Succinicacid
(BSA)BovineSerumAlbumin |