Synonym:IndoxylSulfateAssayProperties:
RelatedCategories | AminoAcidMetabolismAssayKits,BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites,MetabolismAssayKitsA-Z, |
usage | sufficientfor100 colorimetric?tests |
storagetemp. | −20°C |
Description:
FeaturesandBenefits
CompatIBLewithhigh-throughputhandlingsystems.Canbeadaptedforusewithcuvettes.
Generaldescription
Indican(indoxylsulfate)isaputrefactionproductresultingfrombacterialdeconjugationoftryptophaninthesmallintestine.Indicaniswatersolubleandexcretedthroughurine.Increasesinurineindicancanindicateproteinmaldigestionormalabsortion,ortheovergrowthofgutanaerobicbacteria.Indicanlevelsinurinecanalsoincreaseinresponsetoahighproteindiet.
ThisassayisbasedonanimprovedCurzonandWalshmethodinwhichthereactionofindicanwithp-dimethylaminobenzaldehyderesultsinacolorimetricproductmeasuredat480nm.Thisassayhasalineardetectionrangebetween0.9-200mg/dLinthe96wellplateassay.
SuitABIlity
TheIndicanAssaykitprovidesasimpleanddirectprocedureformeasuringindicanlevelsinurine.
Safety:
Symbol
GHS07
Hazardstatements
H315-H319-H335
Precautionarystatements
P261-P305+P351+P338