Superior transient transfection for high yield baculovirus titers in insect cells. Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression. TransIT?-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types that offers exceptional DNA delivery in insect cell types including Sf9, High Five? and S2.