| Description: | GreenView? Plus is a highly sensitive fluorescent stain for detecting nucleic acids in agarose and polyacrylamide gels. This single stain gives high sensitivity detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or alternatively the stain can be added to agarose gels during gel casting. GreenView? Plus is compatible with a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, or a gel reader equipped with visible light excitation such as a 488 nm laser-based gel scanner. GreenView? Plus DNA Gel Stain, 10,000X is a concentrated GreenView Plus solution that can be diluted 10,000 times for use in precast gel staining or 5,000 times for use in post gel staining according to the procedures described below. One vial of 10,000X solution can be used to prepare at least 100 precast minigels or post-stain at least 100 minigels. Gel staining with GreenView? Plus is compatible with downstream applications such as gel extraction and cloning. GreenView Plus is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation. |
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