The microRNA Marker is a set of three synthetic single-stranded RNA oligonucleotides 17, 21 and 25 residues long that have free 5´ ends (i.e., no 5´ phosphate groups). These oligonucleotides can be used as size markers on denaturing polyacrylamide gels and Northern blots. The microRNA Marker is best visualized by staining with SYBR-Gold instead of ethidium bromide (Figure 1).The three marker oligos contain the same core sequence so they can be detected by hybridization with the same probe. The sequences of the microRNA marker band are as follows:25-mer: 5´AGAGCAGUGGCUGGUUGAGAUUUAA 3´ 21-mer: 5´AGCAGUGGCUGGUUGAGAUUU 3´ 17-mer: 5´CAGUGGCUGGUUGAGAU 3´ Note: The sequence in bold is common to all three oligos.A 21-mer DNA oligonucleotide complementary to the marker sequences is included. This oligonucleotide is biotinylated at the 3´ end and has a free 5´ end so it can also be labeled with γ-32P-ATP and T4 Polynucleotide Kinase (NEB# M0201). The sequence of the oligonucleotide probe is as follows:5´AAATCTCAACCAGCCACTGCT 3´-BiotinThe microRNA Marker is provided in a ready-to-load solution containing 4M urea and 0.04% Orange-G. The microRNA Marker Probe is resuspended in water.Figure 1: Lane A: 60 ng of microRNA Marker(5 μl) was loaded on a 12% denaturing polyacrylamide-urea gel which was stained with SYBR Gold (Molecular Probes) for 5 minutes and photographed on a transilluminator. Lane B: 0.2 μg of the Low Range ssRNA Marker (NEB #N0364).
This product is related to the following categories:
miRNA & siRNA Products,
RNA Markers & Ladders Products,
RNA Markers & Ladders Products
This product can be used in the following applications:
購物車為空，快去購物吧！
4000-520-616
咨詢顧問
特別 提示
