Synonym:Acyl-neuraminylHydrolase,Receptor-destroyingenzyme,SialidaseProperties:
RelatedCategories | 3.2.x.xGlycosidases,3.x.x.xHydrolases,ApplicationIndex,BiochemicalsandReagents,CarbohydrateHydrolysis, |
QualityLevel | PREMIUM |
type | TypeIII |
form | bufferedaqueoussolution |
FeaturedIndustry | DiagnosticAssayManufacturing |
foreignactivity | ProteaseandNAN-aldolase,present |
storagetemp. | 2-8°C |
Description:
Application
Neurminidaseisusedasacell-surfaceprobeforglycoconjugatedistributionandinsubstratespecificitystudies.
NeuraminidasefromVibriocholeraehasbeenusedinastudytoassessitsroleinthebindinganduptakeofcholeratoxinbysusceptIBLecells.Ithasalsobeenusedinastudytoinvestigatethepreparationofasodiumsubstrateanditsuseinafluorometricassayofneuraminidase.
Generaldescription
Neuraminidaseenzymesareglycosidehydrolaseenzymesthatcatalyzehydrolysisofterminalsialicacidresidues.Themostwell-knownaretheviralnearamidases,whichpromoteinfluenzavirusrelease.
Packaging
1unitinpolybottle
2unitsinpolybottle
PreparationNote
Chromatographicallypurified.
Quality
Preservativefree.
UnitDefinition
Oneunitwillliberate1.0μmoleofN-acetylneuraminicacidperminatpH5.0at37°CusingNAN-lactoseorbovinesuBMAxillarymucin,unlessotherwisespecified.PricesbasedonunitsusingNAN-lactoseassubstrate.
Physicalform
Aqueoussolution,pH5.5,containing0.15MNaCland4mMCaCl2.
Biochem/physiolActions
Vibriocholeraeneuraminidasehasbeenshowntopromotecholeratoxininfectionbybindingthetoxinandaidinginitsuptakebycells.
Safety:
PersonalProtectiveEquipment
Eyeshields,Gloves,typeN95(US),typeP1(EN143)respiratorfilter
RIDADR
NONHforallmodesoftransport