Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | PP2A-Calpha/beta(Phospho-Tyr307)Antibody |
Synonyms | Serine/threonine-proteinphosphatase2Acatalyticsubunitalpha/betaisoform; |
Immunogen | Theantiserumwasproducedagainstsynthesizedphospho-peptidederivedfromhumanPP2A-Calpha/betaaroundthephosphorylationsiteofTyr307. |
ImmunogenRange | |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000ELISA:1:10000 |
Specificity | PP2A-Calpha/beta(Phospho-Tyr307)antibodydetectsendogenouslevelsofPP2A-Calpha/betaonlywhenphosphorylatedatTyr307. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(Phospho-withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | PPP2CA/PPP2CB |
DatabaseLinks | GeneID: 5515/5516 Swiss-Prot#: P67775/P62714 OMIM#: 176915/176916 Unigene#: Hs.483408/Hs.491440 |
TargetModification | Phospho |
ModificationSites | H:Y307,M:Y307,R:Y307 |
ApplicationImages |
Image1 | Enzyme-LinkedImmunosorbentAssay(Phospho-ELISA)forImmunogenPhosphopeptide(Phospho-left)andNon-Phosphopeptide(Phospho-right),usingPP2A-Cα/β(Phospho-Tyr307)Antibody |
Image2 | WesternblotanalysisoflysatesfromA549cells,usingp-PP2A-Cα/β(Phospho-Tyr307)antibody. |