The antiserum was produced against synthesized phospho-peptide derived from human Pragmin around the phosphorylation site of Tyr413.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Pragmin (Phospho-Tyr413) antibody detects endogenous levels of Pragmin only when phosphorylated at Tyr413.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SGK223
Database Links
Gene ID: 157285 Swiss-Prot #: Q86YV5
OMIM #: None Unigene #: Hs.657673
Target Modification
Phospho
Modification Sites
H:Y413, M:Y196, R:Y391
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Pragmin (Phospho-Tyr413) Antibody
Image 2
Western blot analysis of lysate from A549 cells, using phospho-Pragmin (Phospho-Tyr413) antibody.