The antiserum was produced against synthesized phospho-peptide derived from human AKT1 around the phosphorylation site of Thr308.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
AKT1 (Phospho-Thr308) Antibody detects endogenous levels of AKT1 only when phosphorylated at Thr308.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AKT1
Database Links
Gene ID: 207 Swiss-Prot #: P31749
OMIM #: 164730 Unigene #: Hs.525622
Target Modification
Phospho
Modification Sites
H:T308, M:T308, R:T308
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AKT1 (Phospho-Thr308) Antibody
Image 2
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 5', using AKT1 (Phospho-Thr308) Antibody.