The antiserum was produced against synthesized phospho-peptide derived from human Caspase 9 around the phosphorylation site of Thr125.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Caspase 9 (Phospho-Thr125) Antibody detects endogenous levels of CASP9 only when phosphorylated at Thr125.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CASP9
Database Links
Gene ID: 842 Swiss-Prot #: P55211
OMIM #: 602234 Unigene #: Hs.329502
Target Modification
Phospho
Modification Sites
H:T125, M:T163, R:T163
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Caspase 9 (Phospho-Thr125) Antibody
Image 2
Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 5'+calyculinA 50ng/ml 5', using Caspase 9 (Phospho-Thr125) Antibody.