The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
C-RAF (Phospho-Ser642) Antibody
Synonyms
C-RAF; C-Raf; CRAF; kinase Raf1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; Raf proto-oncogene serine/threonine-protein kinase; RAF-1; Raf-1; RAF1; Similar to murine leukemia viral (Phospho-V-raf-1) oncogene homolog 1
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human C-RAF around the phosphorylation site of Ser642.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
C-RAF (Phospho-Ser642) Antibody detects endogenous levels of RAF1 only when phosphorylated at Ser642.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RAF1
Database Links
Gene ID: 5894 Swiss-Prot #: P04049
OMIM #: 164760/611553/611554 Unigene #: Hs.159130
Target Modification
Phospho
Modification Sites
H:S642, M:S642, R:S642
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C-RAF (Phospho-Ser642) Antibody
Image 2
Western blot analysis of lysates from K562 and HUVEC cells treated with PMA, using C-RAF (Phospho-Ser642) Antibody.