The antiserum was produced against synthesized phospho-peptide derived from human FLT3 around the phosphorylation site of Tyr969.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
FLT3 (Phospho-Tyr969) Antibody detects endogenous levels of FLT3 only when phosphorylated at Tyr969.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
FLT3
Database Links
Gene ID: 2322 Swiss-Prot #: P36888
OMIM #: 136351 Unigene #: Hs.507590
Target Modification
Phospho
Modification Sites
H:Y969
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FLT3 (Phospho-Tyr969) Antibody
Image 2
Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using FLT3 (Phospho-Tyr969) Antibody.