The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
MAPKAPK-2 (Phospho-Thr222) Antibody
Synonyms
MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; MAPKAPK-2; MAPKAPK2; RPS6KC1; kinase MAPKAPK2
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human MAPKAPK-2 around the phosphorylation site of Thr222.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
MAPKAPK-2 (Phospho-Thr222) Antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at Thr222.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAPKAPK2
Database Links
Gene ID: 9261 Swiss-Prot #: P49137
OMIM #: 602006 Unigene #: Hs.643566
Target Modification
Phospho
Modification Sites
H:T222, M:T222, R:T222
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAPKAPK-2 (Phospho-Thr222) Antibody
Image 2
Western blot analysis of lysates from COS7 cells treated with UV 15', using MAPKAPK-2 (Phospho-Thr222) Antibody.