The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
MAPKAPK5 (Phospho-Thr182) Antibody
Synonyms
MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPK5; MAPKAP kinase 5; p38-regulated/activated protein kinase; PRAK
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human MAPKAPK5 around the phosphorylation site of Thr182.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
MAPKAPK5 (Phospho-Thr182) Antibody detects endogenous levels of MAPKAPK5 only when phosphorylated at Thr182.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAPKAPK5
Database Links
Gene ID: 8550 Swiss-Prot #: Q8IW41
OMIM #: 606723 Unigene #: Hs.413901
Target Modification
Phospho
Modification Sites
H:T182, M:T182
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAPKAPK5 (Phospho-Thr182) Antibody
Image 2
Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using MAPKAPK5 (Phospho-Thr182) Antibody.