The antiserum was produced against synthesized phospho-peptide derived from human TAK1 around the phosphorylation site of Thr184.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
TAK1 (Phospho-Thr184) Antibody detects endogenous levels of MAP3K7 only when phosphorylated at Thr184.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP3K7
Database Links
Gene ID: 6885 Swiss-Prot #: O43318
OMIM #: 602614 Unigene #: Hs.714773
Target Modification
Phospho
Modification Sites
H:T184, M:T184, R:T184
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TAK1 (Phospho-Thr184) Antibody
Image 2
Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using TAK1 (Phospho-Thr184) Antibody.