The activity is determined by the dose-dependant proliferation of the mouse FDC-P1 cell line. The expected ED50 for this effect is 0.9-1.3 pg/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie BlueLane 1: non-reducing conditionsLane 2: reducing conditions
Storage Buffer:
Lyophilized from 20 mM NaHCO3
Storage Instruction:
Store at -20°C on dry atmosphere.After reconstitution with sterilized water, store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of rat Csf2, starting at 10 ng/mL, were added to FDCP-1 cells. After 91 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50.