Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug).
Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 0.01-1.0 ug/ml.
Recommend Usage:
Activity assaySDS-PAGEThe optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 5 mM Sodium Phosphate (pH 7.0, 0.1 mM Calcium Chloride).
Storage Instruction:
Store at -20°C.Reconstitute in water to a concentration of 0.5-1.0 mg/mL. Do not vortex.For extended storage, it is recommended to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.