α-Lytic Protease (aLP) cleaves after Threonine (T), Alanine (A), Serine (S) and Valine (V) residues. Its specificity makes it an orthogonal and alternative protease to others commonly used in proteomics applications, including trypsin and chymotrypsin. Peptides generated by aLP are of similar average length to those of Trypsin.
Figure 1. α-Lytic Protease can be used alone or in combination with other proteases to yield increased sequence coverage.Comparison of sequence coverage for three protein standards after parallel digestion using Trypsin (blue) and a-Lytic Protease (gold). The combined data set (grey) results in overlapping peptides and increased sequence coverage.
Product Source
Purified from Lysobacter enzymogenes
This product is related to the following categories:
Proteases Products,
Proteome Analysis Products
This product can be used in the following applications: