Rabbit polyclonal antibody raised against full length recombinant SARS orf1ab.
Immunogen:
Recombinant protein corresponding to amino acids 834-943 of ADRP region, 1208-1557 of SARS-Unique region and 1558-2040 of Papain-like proteinase region of SARS-CoV orf1ab.
Host:
Rabbit
Form:
Liquid
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Recommend Usage:
Western Blot (1:1000)Immunofluorescence (1:1000)Immunoprecipitation (1:60)Immunoelectron Microscopy (1:500)The optimal working dilution should be determined by the end user.
Storage Buffer:
In antiserum (0.01% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJ, van der Meulen J, Koerten HK, Mommaas AM.J Virol. 2006 Jun;80(12):5927-40.
2.
ADP-ribose-1"-monophosphatase: a conserved coronavirus enzyme that is dispensable for viral replication in tissue culture.Putics A, Filipowicz W, Hall J, Gorbalenya AE, Ziebuhr J.J Virol. 2005 Oct;79(20):12721-31.
3.
Identification of severe acute respiratory syndrome coronavirus replicase products and characterization of papain-like protease activity.Harcourt BH, Jukneliene D, Kanjanahaluethai A, Bechill J, Severson KM, Smith CM, Rota PA, Baker SC.J Virol. 2004 Dec;78(24):13600-12.
Applications
Immunofluorescence
Immunofluorescence Microscopy using orf1ab polyclonal antibody (Cat # PAB11371), 6 hrs post infection Vero-E6 cells.Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.