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Abnova/CD274 polyclonal antibody/1kit/PAB12985
  • Abnova/CD274 polyclonal antibody/1kit/PAB12985

Abnova/CD274 polyclonal antibody/1kit/PAB12985

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貨號: PAB12985
品牌: Abnova
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  • 詳情
  • 使用說明
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      • Specification
      • Product Description:
      • Rabbit polyclonal antibody raised against synthetic peptide of CD274.
      • Immunogen:
      • A synthetic peptide corresponding to internal region 17 amino acids of human CD274.
      • Host:
      • Rabbit
      • Reactivity:
      • Human, Mouse, Rat
      • Specificity:
      • Has no cross-reactivity to CD273.
      • Form:
      • Liquid
      • Purification:
      • Peptide affinity purification
      • Isotype:
      • IgG
      • Recommend Usage:
      • Flow Cytometry (0.5 ug/mL)Immunofluorescence (20 ug/mL)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL)Western Blot (0.5-1 ug/mL)The optimal working dilution should be determined by the end user.
      • Storage Buffer:
      • In PBS (0.02% sodium azide).
      • Storage Instruction:
      • Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
      • Note:
      • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • Hepatic Artery Embolization Enhances Expression of Programmed Cell Death 1 Ligand 1 in an Orthotopic Rat Hepatocellular Carcinoma Model: In Vivo and in Vitro Experimentation.Haruyuki Takaki, Yutaka Hirata, Eisuke Ueshima, Hiroshi Kodama, Seiji Matsumoto, Reona Wada, Hitomi Suzuki, Keiji Nakasho, Koichiro Yamakado.J Vasc Interv Radiol. 2020 Sep;31(9):1475-1482.e2. doi: 10.1016/j.jvir.2020.03.023. Epub 2020 Aug 14.
      • 2.
      • Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1).LaGier AJ, Pober JS.Hum Immunol. 2006 Aug;67(8):568-78. Epub 2006 May 19.
      • 3.
      • Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men.Holling TM, Schooten E, van Den Elsen PJ.Hum Immunol. 2004 Apr;65(4):282-90.
      • 4.
      • Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.Ishida Y, Agata Y, Shibahara K, Honjo T.EMBO J. 1992 Nov;11(11):3887-95.
      • Applications
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • Western blot analysis of CD274 in Raji cell lysate with CD274 polyclonal antibody (Cat # PAB12985) at (A) 0.5 and (B) 1 ug/mL .
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry of CD274 in human heart tissue with CD274 polyclonal antibody (Cat # PAB12985) at 2.5 ug/mL .
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence staining of human heart cells with CD274 polyclonal antibody (Cat# PAB12985) under 20 ug/mL working concentration.
      • Enzyme-linked Immunoabsorbent Assay
      • Flow Cytometry
      • Flow Cytometry
      • Flow cytometric analysis of A-20 cells with CD274 polyclonal antibody (Cat# PAB12985). Green: Isotype control. Red : CD274 antibody.
      • Application Image
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • enlarge
      • Immunofluorescence
      • Immunofluorescence
      • enlarge
      • Enzyme-linked Immunoabsorbent Assay
      • Flow Cytometry
      • Flow Cytometry
      • enlarge
      • Gene Information
      • Entrez GeneID:
      • 29126
      • Protein Accession#:
      • NP_054862
      • Gene Name:
      • CD274
      • Gene Alias:
      • B7-H,B7H1,MGC142294,MGC142296,PD-L1,PDCD1L1,PDCD1LG1,PDL1
      • Gene Description:
      • CD274 molecule
      • Omim ID:
      • 605402
      • Gene Ontology:
      • Hyperlink
      • Other Designations:
      • CD274 antigen,OTTHUMP00000021029,programmed cell death 1 ligand 1
      • Gene Pathway
      • Cell adhesion molecules (CAMs)
      • Related Disease
      • Addison Disease
      • Arthritis, Rheumatoid
      • Autoimmune Diseases
      • Diabetes Mellitus, Type 1
      • Genetic Predisposition to Disease
      • Graves Disease
      • Lupus Erythematosus, Systemic
      • Multiple Sclerosis
      • Narcolepsy
      • Rheumatoid Nodule
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