Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1.
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Y412 of human ABL1.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody detects endogenous levels of ABL1 only when phosphorylated at tyrosine 412.
Form:
Liquid
Recommend Usage:
Western Blot (1:500-1:1000)Immunohistochemistry (1:50-1:100)ELISA (1:4000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.Rikova K, Guo A, Zeng Q, Possemato A, Yu J, Haack H, Nardone J, Lee K, Reeves C, Li Y, Hu Y, Tan Z, Stokes M, Sullivan L, Mitchell J, Wetzel R, Macneill J, Ren JM, Yuan J, Bakalarski CE, Villen J, Kornhauser JM, Smith B, Li D, Zhou X, Gygi SP, Gu TL, Polakiewicz RD, Rush J, Comb MJ.Cell. 2007 Dec 14;131(6):1190-203.
2.
Autoinhibition of c-Abl.Pluk H, Dorey K, Superti-Furga G.Cell. 2002 Jan 25;108(2):247-59.
3.
JAK-STAT signaling activated by Abl oncogenes.Danial NN, Rothman P.Oncogene. 2000 May 15;19(21):2523-31.
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells treated with Adriamycin.Lane 1 : Using ABL1 (phospho Y412) polyclonal antibody (Cat # PAB16947).Lane 2 : Using the same antibody preincubated with synthesized peptide.
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response.Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene.The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia.The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq
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