描述
qubit?(早期稱為Quant-iT?)dsDNABR分析試劑盒(Qubit?dsDNABRAssayKit)專門設計用于在Qubit?2.0熒光儀(Q32866),但也可以在任何熒光計或熒光酶標儀中使用。該試劑盒對雙鏈DNA(dsDNA)有高度的選擇性,不會定量RNA,可以定量濃度為100pg/μl到1000ng/μl的樣品。試劑盒提供了濃縮的分析試劑,稀釋緩沖液和預制的DNA標準。您只需用提供的緩沖液稀釋試劑,添加樣品(體積在1μL到20μL范圍內(nèi)),然后使用Qubit?2.0熒光儀讀取濃度。該試劑盒對于常見污染物,如鹽,游離的核苷酸,溶劑,去污劑,蛋白質(zhì)的耐受性極好。
注意:所有的Qubit?分析試劑盒同樣適用于Qubit?1.0熒光儀。
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Description
TheQubitdsDNABRAssayKitisdesignedspecificallyforusewiththeQubitFluorometer.Theassayishighlyselectivefordouble-strandedDNA(dsDNA)overRNAandisdesignedtobeaccurateforinitialsampleconcentrationfrom100pg/μl–1,000ng/μl.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedDNAstandards.Simplydilutethereagentusingthebufferprovided,addyoursample(anyvolumebetween1μland20μlisacceptable),andreadtheconcentrationusingtheQubitFluorometer.Commoncontaminants,suchassalts,freenucleotides,solvents,detergents,orproteinarewelltoleratedintheassay.
WhichproducttochoosefordsDNABR(BroadRange)quantitation?
?For1–20samples:usethisQubitdsDNABRAssayKitwiththeQubitFluorometer
?For20–2000samples:usetheQuant-iTdsDNABRAssayKitwithmicroplatereader
Notes:
1.AllQubitassaykitscanbeusedwiththeQubit1.0,Qubit2.0,Qubit3,andQubit4fluorometers.
2. 500μLthin-walledPCRtubes arerequiredbutnotincluded.ForResearchUseOnly.Notforuseindiagnosticprocedures.
Specifications
SampleType(General): | dsDNA |
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ForUseWith(Equipment): | Qubit?Fluorometer |
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QuantitationRange: | 2-1000ng |
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NumberofReactions: | 100reactions |
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ProductLine: | Quant-iT?, Qubit? |
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ProductSize: | 100assays |
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ShippingCondition: | RoomTemperature |
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Contents&storage
Storeinrefrigerator(2–8°C)andprotectfromlight.