qubit?(早期稱為Quant-iT?)dsDNAHS分析試劑盒(Qubit?dsDNAHSAssayKit)專門設(shè)計(jì)用于在Qubit?3.0熒光儀(Q32866),但也可以在任何熒光計(jì)或熒光酶標(biāo)儀中使用。該試劑盒對(duì)雙鏈DNA(dsDNA)有高度的選擇性,不會(huì)定量RNA,可以定量濃度為10pg/μl到100ng/μl的樣品。試劑盒提供了濃縮的分析試劑,稀釋緩沖液和預(yù)制的DNA標(biāo)準(zhǔn)。您只需用提供的緩沖液稀釋試劑,添加樣品(體積在1μL到20μL范圍內(nèi)),然后使用Qubit?3.0熒光儀讀取濃度。該試劑盒對(duì)于常見(jiàn)污染物,如鹽,游離的核苷酸,溶劑,去污劑,蛋白質(zhì)的耐受性極好。
注意:所有的Qubit?分析試劑盒同樣適用于Qubit?3.0熒光儀。
Description
TheQubitdsDNAHS(HighSensitivity)AssayKitisdesignedspecificallyforusewiththeQubitFluorometer.Theassayishighlyselectivefordouble-strandedDNA(dsDNA)overRNAandisdesignedtobeaccurateforinitialsampleconcentrationsfrom10pg/μLto100ng/μL.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedDNAstandards.Simplydilutethereagentusingthebufferprovided,addyoursample(anyvolumebetween1μland20μlisacceptable),andreadtheconcentrationusingtheQubitFluorometer.Commoncontaminants,suchassalts,freenucleotides,solvents,detergents,orproteinarewelltoleratedintheassay.
WhichproducttochoosefordsDNAHS(HighSensitivity)quantitation?
?For1–20samples:usethisQubitdsDNAHSAssayKitwiththeQubitFluorometer
?For20–2000samples:usetheQuant-iTdsDNAHSAssayKitwithmicroplatereader
Notes:
1.AllQubitassaykitscanbeusedwiththeQubit1.0,Qubit2.0,Qubit3,andQubit4fluorometers.
2. 500μLthin-walledPCRtubes arerequiredbutnotincluded.
3.Alsoavailableasa"1X"dsDNAassaykitthatprovidesreagentandbufferinaformulationthatisstableasaready-to-usesolutionforuptooneyearafterpreparation.
ForResearchUseOnly.Notforuseindiagnosticprocedures.