PstI has a High Fidelity version PstI-HF® (NEB #R3140).High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
A E. coli strain that carries the PstI gene from Providencia stuartii 164 (ATCC 49762).
This product is related to the following categories:
Restriction Endonucleases P R,
Time-Saver Qualified Restriction Enzymes Products
This product can be used in the following applications:
BioBrick® Assembly,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB