Description:
PvuIIhasaHighFidelityversionPvuII-HF?(NEB#R3151).HighFidelity(HF?)RestrictionEnzymeshave100%activityinCutSmart?Buffer;single-buffersimplicitymeansmorestraightforwardandstreamlinedsampleprocessing.?HFenzymesalsoexhibitdramaticallyreducedstaractivity.?HFenzymesareallTime-Saver??qualifiedandcanthereforecutsubstrateDNAin5-15minuteswiththeflexibilitytodigestovernightwithoutdegradationtoDNA.?Engineeredwithperformanceinmind,HFrestrictionenzymesarefullyactiveunderabroaderrangeofconditions,minimizingoff-targetproducts,whileofferingflexibilityinexperimentaldesign.?
ProductSource
AnE.colistrainthatcarriesthePvuIIgenefromProteusvulgaris(ATCC13315).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBuffer™3.1 | -20 | 10X |
Notes:
MayexhibitstaractivityinCutSmartBuffer.PvuIIdoesnotcutCAGm4CTG,whilecuttingatCAGm5CTGoccursatasignificantlyreducedrate.Forenzymesthatcannotbeheat-inactivated,werecommendusingacolumnforcleanup(suchastheMonarch?PCR&DNACleanupKit),orrunningthereactiononanagarosegelandthenextractingtheDNA(werecommendMonarchGelExtractionKit),orperformingaphenol/chloroformextraction.